Chromatin Immunoprecipitation:Article Title: Harnessing 3D collagen hydrogel-directed conversion of human GMSCs into SCP-like cells to generate functionalized nerve conduits
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used For IF: mouse anti-p75 (5 μg/mL, Sigma, St. Louis, Missouri, USA), rabbit anti-SOX-9 (5μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-HES1 (5μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), mouse anti-SOX10 (5μg/mL, R & D System, Minneapolis, Minnesota, USA), rabbit anti-S-100beta (5μg/mL, Boster Biological Tech, Pleasanton, California), rabbit antiNotch3 (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), rabbit anti-BDNF (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), rabbit anti-GDNF (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), rabbit anti-NGF (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), mouse anti-vinculin (5 μg/mL, Sigma, St. Louis, Missouri, USA), TRITC-phalloidin (0.5 μg/mL, Sigma, St. Louis, Missouri, USA), mouse anti-neurofilament (5μg/mL, BioLegend, San Diego, California, USA), goat anti-rabbit IgG, Alexa Fluor 488, goat anti-rabbit IgG, Alexa Fluor 594, goat anti-mouse IgG, Alexa Fluor 488, goat anti-mouse IgG, Alexa Fluor 594, or normal anti-rabbit, normal anti-mouse IgG, Alexa Fluor 488 , Alexa Fluor 594 (2.5 μg/mL, BioLegend, San Diego, California, USA). .. For Western blot: rabbit anti-p75 (1μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-HES1 (1μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-GAPDH (1 μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-Notch3 (1μg/mL, Abcam, Cambridge, Massachusetts, USA).
Flow Cytometry:Article Title: Harnessing 3D collagen hydrogel-directed conversion of human GMSCs into SCP-like cells to generate functionalized nerve conduits
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used For IF: mouse anti-p75 (5 μg/mL, Sigma, St. Louis, Missouri, USA), rabbit anti-SOX-9 (5μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-HES1 (5μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), mouse anti-SOX10 (5μg/mL, R & D System, Minneapolis, Minnesota, USA), rabbit anti-S-100beta (5μg/mL, Boster Biological Tech, Pleasanton, California), rabbit antiNotch3 (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), rabbit anti-BDNF (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), rabbit anti-GDNF (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), rabbit anti-NGF (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), mouse anti-vinculin (5 μg/mL, Sigma, St. Louis, Missouri, USA), TRITC-phalloidin (0.5 μg/mL, Sigma, St. Louis, Missouri, USA), mouse anti-neurofilament (5μg/mL, BioLegend, San Diego, California, USA), goat anti-rabbit IgG, Alexa Fluor 488, goat anti-rabbit IgG, Alexa Fluor 594, goat anti-mouse IgG, Alexa Fluor 488, goat anti-mouse IgG, Alexa Fluor 594, or normal anti-rabbit, normal anti-mouse IgG, Alexa Fluor 488 , Alexa Fluor 594 (2.5 μg/mL, BioLegend, San Diego, California, USA). .. For Western blot: rabbit anti-p75 (1μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-HES1 (1μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-GAPDH (1 μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-Notch3 (1μg/mL, Abcam, Cambridge, Massachusetts, USA).
Magnetic Resonance Imaging:Article Title: Harnessing 3D collagen hydrogel-directed conversion of human GMSCs into SCP-like cells to generate functionalized nerve conduits
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used For IF: mouse anti-p75 (5 μg/mL, Sigma, St. Louis, Missouri, USA), rabbit anti-SOX-9 (5μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-HES1 (5μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), mouse anti-SOX10 (5μg/mL, R & D System, Minneapolis, Minnesota, USA), rabbit anti-S-100beta (5μg/mL, Boster Biological Tech, Pleasanton, California), rabbit antiNotch3 (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), rabbit anti-BDNF (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), rabbit anti-GDNF (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), rabbit anti-NGF (2.5μg/mL, Abcam, Cambridge, Massachusetts, USA), mouse anti-vinculin (5 μg/mL, Sigma, St. Louis, Missouri, USA), TRITC-phalloidin (0.5 μg/mL, Sigma, St. Louis, Missouri, USA), mouse anti-neurofilament (5μg/mL, BioLegend, San Diego, California, USA), goat anti-rabbit IgG, Alexa Fluor 488, goat anti-rabbit IgG, Alexa Fluor 594, goat anti-mouse IgG, Alexa Fluor 488, goat anti-mouse IgG, Alexa Fluor 594, or normal anti-rabbit, normal anti-mouse IgG, Alexa Fluor 488 , Alexa Fluor 594 (2.5 μg/mL, BioLegend, San Diego, California, USA). .. For Western blot: rabbit anti-p75 (1μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-HES1 (1μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-GAPDH (1 μg/mL, Cell Signaling Tech, Danvers, Massachusetts, USA), rabbit anti-Notch3 (1μg/mL, Abcam, Cambridge, Massachusetts, USA).
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